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Image Search Results
Journal: Journal of Physiological Anthropology
Article Title: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots
doi: 10.1186/s40101-020-00228-8
Figure Lengend Snippet: Thyroid peroxidase autoantibodies (TPO-Ab) are detectable by enzyme-linked immunoassay in 3-mm punches taken from dried blood spot samples. Washed red blood cells were spiked with Liquichek Speciality Immunoassy Control (levels 1, 2, or 3) and then dropped on Whatman 903 Protein Saver filter paper cards. Cards were dried for 4 h and then frozen at − 80 °C. Levels of TPO-Ab in one 3-mm punch taken from these cards were quantified using the Accubind anti-thyroid peroxidase test system (Cat.#1125-300, Monobind Inc.). RBCs, red blood cells; L1, L2, L3; levels 1, 2, and 3 of the Liquichek Speciality Immunoassy Control, respectively
Article Snippet: Of the 182 plasma samples in our validation set, 12 were positive for TPO-Ab (> 40 IU/mL) (6.6% of our sample population), 144 samples had undetectable levels of TPO-Ab (78.3%), and 28 samples (15.2%) had
Techniques:
Journal: Journal of Physiological Anthropology
Article Title: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots
doi: 10.1186/s40101-020-00228-8
Figure Lengend Snippet: Sensitivity and specificity of the in-house TPO-Ab ELISA for 182 matched plasma, venous DBS (vDBS), and fingerprick DBS (fDBS) samples
Article Snippet: Of the 182 plasma samples in our validation set, 12 were positive for TPO-Ab (> 40 IU/mL) (6.6% of our sample population), 144 samples had undetectable levels of TPO-Ab (78.3%), and 28 samples (15.2%) had
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Journal of Physiological Anthropology
Article Title: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots
doi: 10.1186/s40101-020-00228-8
Figure Lengend Snippet: Dilutional linearity of the in-house TPO-Ab ELISA for dried blood spots
Article Snippet: Of the 182 plasma samples in our validation set, 12 were positive for TPO-Ab (> 40 IU/mL) (6.6% of our sample population), 144 samples had undetectable levels of TPO-Ab (78.3%), and 28 samples (15.2%) had
Techniques: Enzyme-linked Immunosorbent Assay
Journal: Journal of Physiological Anthropology
Article Title: Development and validation of an ELISA for a biomarker of thyroid dysfunction, thyroid peroxidase autoantibodies (TPO-Ab), in dried blood spots
doi: 10.1186/s40101-020-00228-8
Figure Lengend Snippet: a Stability of thyroid peroxidase autoantibodies (TPO-Ab) concentrations in response to storage over time at different temperatures. Venous DBS (vDBS) samples from four individuals were stored at − 28 °C, at room temperature (RT, ~22 °C), and at 37 °C for 28 days. On days 2, 7, 14, and 28 of storage, samples were transferred to − 80 °C. TPO-Ab concentrations in these samples were compared to those in samples stored at − 80 °C after collection and thawed only once for this experiment. The average percentage recovery for all four samples at the various timepoints for the three different storage temperatures is shown. Error bars are standard errors of the mean. Dotted lines indicate 80 and 120% recovery. b Stability of TPO-Ab concentrations in response to the number of freeze-thaw cycles. vDBS samples from six individuals were stored at − 28 °C and then allowed to thaw at RT for 2 h two, four, eight, or twelve times (each on separate days) after which they were returned to the − 28 °C freezer. These samples were then thawed one final time to assay TPO-Ab concentrations, for a total number of freeze-thaw cycles of 3, 5, 8, 9, and 13, respectively. TPO-Ab concentrations in these samples were compared to those in samples stored at − 80 °C after collected and thawed only once for this experiment. Dotted lines indicate 80 and 120% recovery
Article Snippet: Of the 182 plasma samples in our validation set, 12 were positive for TPO-Ab (> 40 IU/mL) (6.6% of our sample population), 144 samples had undetectable levels of TPO-Ab (78.3%), and 28 samples (15.2%) had
Techniques: